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Antioxidant and Toxicity Assay-guided Isolation of Herniarin from Equisetum debile Roxb. (Equisetaceae)

1, 2, 2

  1. 1Department of Chemistry, College of Arts and Sciences, Central Mindanao University, Musuan, Bukidnon, PHILIPPINES.
  2. 2Department of Chemistry, College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, A. Bonifacio Ave, Tibanga, Iligan City, PHILIPPINES.

CORRESPONDENCE

Aileen May Gabule Ang

Department of Chemistry, College of Arts and Sciences, Central Mindanao University, Musuan, Bukidnon, PHILIPPINES.

aisemg_ang@yahoo.com

Received: 22-01-2019; Accepted: 12-04-2019.

Volume 8, Issue 1 · pp. 30-35 · 2019 · DOI: 10.5530/ajbls.2019.8.5

ABSTRACT

Introduction: Bioassay-guided isolation of the bioactive components from Equisetum debile Roxb. (“sumbak”) was conducted.Objectives: This study aimed to isolate and characterize bioactive components from E. debile.Materials and Methods: Ethanolic extract of whole plant sample of E. debile was sequentially partitioned to obtain the bioactive chloroform-soluble (EdC) partition. EdC was purified via chromatographic techniques. Fractions obtained after purification were analyzed for cytotoxic and antioxidant properties. Brine shrimp lethality as well as in vitro antioxidant assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Total Antioxidant Capacity and Total Phenolics Content assays were carried out. One-Dimensional (1D) and two-dimensional NMR and LC-MS analyses were conducted to elucidate the structure of the isolated compound. Results and Discussion: The whole plant sample of E. debile afforded herniarin.Conclusion: The present findings imply that E. debile can be a potential source of bioactive components.

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    Ang, A. M. G., Peteros, N. P., & Uy, M. M. (2019). Antioxidant and Toxicity Assay-guided Isolation of Herniarin from Equisetum debile Roxb. (Equisetaceae). Asian Journal of Biological and Life Sciences, 8(1), 30-35. https://doi.org/10.5530/ajbls.2019.8.5

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    © 2019 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0