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Toxicological Evaluation of Ashtabhairava mathirai Acute and Subacute Safety Assessment

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  1. 1Department of Nanju Maruthuvam (Siddha Toxicology), National Institute of Siddha, (Affiliated with The Tamil Nadu Dr. M. G. R Medical University), Ministry of Ayush, Chennai, Tamil Nadu, INDIA.
  2. 2Department of Gunapadam (Siddha Pharmacology), National Institute of Siddha, (Affiliated with The Tamil Nadu Dr. M. G. R Medical University), Ministry of Ayush, Chennai, Tamil Nadu, INDIA.

CORRESPONDENCE

Dr. Raghavi Marimuthu

Department of Nanju Maruthuvam (Siddha Toxicology), National Institute of Siddha (Affiliated to TN Dr. M.G.R Medical University), Ministry of Ayush, Chennai-47, Tamil Nadu, INDIA.

muthuraghavi92@gmail.com

Received: 12-02-2025; Revised: 08-04-2025; Accepted: 25-06-2025.

Volume 14, Issue 2 · pp. 314-324 · 2025 · DOI: 10.5530/ajbls.20251486

ABSTRACT

Background/Purpose: Ashtabhairava mathirai(ABM), a traditional Siddha formulation referenced in the Siddha Vaidhiya Thirattu, is traditionally used to treat 64 types of fever. Despite its historical significance, scientific validation of its safety is crucial for its acceptance in modern medicine. This study aimed to evaluate the acute and subacute toxicity of ABM using Wistar albino rats (Rattus norvegicus).Materials and Methods: Wistar albino rats were used for acute (OECD 423) and subacute (OECD 407) toxicity studies. Behavioral, biochemical, and histopathological parameters were analyzed. Data were statistically evaluated using one-way ANOVA (p<0.05).Results: The findings of the study demonstrated that all evaluated parameters remained within normal limits, with no mortality observed throughout the experimental period. The acute oral toxicity assessment indicated that the LD50value of Ashtabhairava mathirai(ABM) exceeds 2000 mg/kg body weight. Additionally, the repeated dose 28-day oral toxicity study determined No Observed Adverse Effect Level (NOAEL) of more than 150 mg/kg body weight. Histopathological examinations of vital organs provided further reassurance, as no abnormalities or adverse changes were noted, supporting safety of the formulation.Conclusion: These results confirm the safety of ABM for further clinical investigations but also validate its ongoing use in traditional practices. This study offers a thorough preclinical safety evaluation of ABM, providing scientific support for its traditional applications and potential as a therapeutic agent in future medical use.

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    Marimuthu, R., Balasubramani, A., Ramamoorthy, K., Ramasamy, S., Rajaraman, R., Rajathinakaran, S. A., & Ramachandran, M. (2025). Toxicological Evaluation of Ashtabhairava mathirai Acute and Subacute Safety Assessment. Asian Journal of Biological and Life Sciences, 14(2), 314-324. https://doi.org/10.5530/ajbls.20251486

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    © 2025 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0