Asian Journal of Biological and Life Sciences logo
Submit
Asian Journal of Biological and Life SciencesACADEMIC PUBLISHING
Article

Exploring Mycorrhizal Associations in Orchids: Isolation, Identification, and Morphological Insights

1, 1

  1. 1Department of Biosciences, Chandigarh University, Gharuan, Punjab, INDIA.

CORRESPONDENCE

Prof. Dr. Lucky K Attri

Professor and HoD, Department of Biosciences, Chandigarh University, Gharuan, Punjab, INDIA.

attril@rediffmail.com

Received: 07-03-2025; Revised: 18-04-2025; Accepted: 23-06-2025.

Volume 14, Issue 2 · pp. 309-313 · 2025 · DOI: 10.5530/ajbls.20251487

ABSTRACT

Background/Purpose: Epiphytic orchids, such as Aerides odorata L., rely on symbiotic mycorrhizal fungi for nutrient acquisition, germination, and early growth due to the nutrient-deficient nature of their seeds. The hypothesis of this study was that Rhizoctonia spp. are the primary mycorrhizal fungi associated with A. odorataand that their growth characteristics vary depending on the culture medium.Materials and Methods: To test this, fungal samples were isolated from A. odorataand cultured on four different media: Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA), Malt Extract Agar (MEA), and Czapek Dox Agar (CZA).Results: Microscopic examination confirmed the presence of Rhizoctonia spp., characterized by septate hyphae and the absence of conidia, indicating asexual reproduction. Fungal growth was evaluated, with PDA and SDA showing the most rapid and extensive growth, while CZA exhibited the slowest and most restricted growth.Conclusion: The results suggest that Rhizoctonia spp. play a significant role in the symbiosis of A. odorata L., and that media selection is crucial for optimizing fungal cultivation. These findings contribute to improving ex situ fungal culture techniques, which are vital for the conservation and sustainable propagation of A. odorata L.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Bhattacharjee, S., & Attri, L. K. (2025). Exploring Mycorrhizal Associations in Orchids: Isolation, Identification, and Morphological Insights. Asian Journal of Biological and Life Sciences, 14(2), 309-313. https://doi.org/10.5530/ajbls.20251487

    Copyright and licence

    © 2025 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0