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Determination of Potential Anti Quorum Sensing Activity of Eleusine indica Ethanolic Crude Extract against Escherichia coli and Staphylococcus aureus

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  1. 1Department of Medical Technology, Institute of Health Sciences and Nursing, Far Eastern University, Manila, Metro Manila, PHILIPPINES.

CORRESPONDENCE

Eziah C. San Pascual

Department of Medical Technology, Institute of Health Sciences and Nursing, Far Eastern University, Manila, Metro Manila, PHILIPPINES.

dbercede@feu.edu.ph

Received: 11-06-2024; Revised: 22-07-2024; Accepted: 22-08-2024.

Volume 13, Issue 2 · pp. 456-465 · 2024 · DOI: 10.5530/ajbls.2024.13.57

ABSTRACT

Aim/Background: The study assesses the potential anti-quorum sensing ability of the ethanolic crude extract of the plant Eleusine indica and its ability to inhibit biofilm formation in two clinically significant bacteria, Escherichia coli and Staphylococcus aureus.Materials and Methods: To prove its anti-quorum sensing capability, antimicrobial susceptibility testing was done by placing filter paper disks loaded with Eleusine indica with varying concentrations (25%, 50%, 75%, and 100%) on a MH agar inoculated with the two bacteria. Subsequently, biofilm inhibition testing was conducted to evaluate the extract’s effect on the bacteria’s biofilm formation by combining the Eleusine indica crude extract with overnight cultures of the bacteria in a 96-well plate, then measuring its optical density. Descriptive statistics were employed to analyze the optical density data, with values higher than the positive control indicating increased biofilm formation.Results: Antimicrobial susceptibility testing showed that no antimicrobial activity was present in the extract for the two bacteria. Moreover, results revealed that Eleusine indica crude extract did not inhibit biofilm formation in gram-positive Staphylococcus aureus, while all concentrations (25%, 50%, 75%, and 100%) successfully inhibited biofilm formation in gram-negative Escherichia coli.Conclusion: These findings suggest that Eleusine indica may possess anti-quorum sensing properties against certain bacteria. Further research on the overall mechanism of Eleusine indica and its differential response on different clinically significant bacteria is warranted.

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    Pascual, E. C. S., Jose, M. A. T. S., Santos, A. M. C., Tinte, T. J. E., Trinidad, J. L., Vidal, A. A. D., Zabala, L. J. C., Zapanta, R. C. C., & Bercede, D. H. (2024). Determination of Potential Anti Quorum Sensing Activity of Eleusine indica Ethanolic Crude Extract against Escherichia coli and Staphylococcus aureus. Asian Journal of Biological and Life Sciences, 13(2), 456-465. https://doi.org/10.5530/ajbls.2024.13.57

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    © 2024 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0