Investigating the Anti-Biofilm Ability of Synergistic Drug Coated Urinary Catheters: In vitro Study
1, 1
- 1Department of Microbiology, Nehru Arts and Science College Coimbatore, Tamil Nadu, INDIA.
CORRESPONDENCE
Naveena Rajasree R
Department of Microbiology, Nehru Arts and Science College Coimbatore, Tamil Nadu, INDIA.
Received: 24-05-2024; Revised: 19-07-2024; Accepted: 25-08-2024.
Volume 13, Issue 2 · pp. 368-375 · 2024 · DOI: 10.5530/ajbls.2024.13.47
ABSTRACT
Aim: Coating the surface of a urinary catheter using two different synergistic drug combinations to prevent bacterial adhesion was considered the primary objective of the study.Materials and Methods: Coatings were carried out using two steps: seeding and crystallization with Polyethylene Glycol (PEG) as a binding agent. Coated samples were analyzed for FESEM analysis and FTIR analysis. The FTIR spectrum of the synergistic drugs and carrier-coated silicone was analyzed to detect the chemical interactions among them. Finally, an anti-biofilm assay using the minimal biofilm eradication concentration was determined to check the efficacy of the coated samples.Findings: Topographical analysis of the coated samples revealed a large, uniform, and continuous layer of parallelogram shapes on the catheter surface. The FTIR spectrum showed that the addition of drugs and carriers did not alter the functional group of silicone. C-H vibration for methyl groups of silicone, terminal C-H stretch of drugs, and asymmetric methyl groups of carriers were evident from some of the common peaks. The obtained peaks showed that the functional group of silicone was not altered.Conclusion: These in vitro outcomes suggest that the antimicrobial combinations used in the present research (Cefixime + Ciprofloxacin; and Ofloxacin and Ornidazole) with specific carriers (tocopherol acetate and beta cyclodextrin) can possibly be used to combat bacterial colonization and catheter-associated infections.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
R, N. R., & M, T. (2024). Investigating the Anti-Biofilm Ability of Synergistic Drug Coated Urinary Catheters: In vitro Study. Asian Journal of Biological and Life Sciences, 13(2), 368-375. https://doi.org/10.5530/ajbls.2024.13.47
Copyright and licence
© 2024 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0