Plant Growth Promotion in Solanum lycopersicum through Endophytic bacteria Isolated from Flower of Samanea saman

ABSTRACT

Aim: Plant Growth Promotion in Solanum lycopersicum through Endophytic bacteria isolated from Flower of Samanea saman and antifungal activity against Fusarium oxysporum.Materials and Methods: Endophytes were isolated from the Rain Tree Flower samples collected from Sadakathullah Appa College Campus, Rahmath nagar, Tirunelveli, Tamil Nadu, India and assessed for their protective activity against Fusarium oxysporum, which causes wilt disease. The flower samples were washed in tap water for removing dirt particles and soaked in 70% ethanol for 3 min; further washed with sodium hypochlorite solution (2.5% available Chlorine) for 5 min and cleaned well with sterile distilled water. Flower samples were crushed with a sterile mortar and pestle and then plated on nutrient agar media. The plates were incubated for 3 to 5 days at 28ºC for bacterial growth. Bacterial strains were tested for antagonism activity against Fusarium oxysporum using Potato dextrose agar. The actively growing fungal (F. oxysporum) mycelial disc (5 mm) was inserted at one side and a loop of endophytic bacteria was streaked in a line of about 6 cm in length. One control plate was also kept for observation. Those plates were incubated at 28±2ºC for 7 days and the inhibition zone was measured. Results and Discussion: In the present study, a total of 2 endophytes SRFB1 and SRFB2 were isolated from the Rain Tree Flower samples; among them, endophyte SRFB1 showed significant results on shoot and root length, fresh and dry weight, respectively, when compared with the control. One of the isolates was considered as an efficient endophytic isolate as it possessed plant growth promotion and SRFB1 showed positive results against Fusarium oxysporum.Conclusion: The present study shows that the endophytes isolated from the flower samples can be commercially formulated as an effective biocontrol agent against soil-borne fungal pathogens which affect plants. In the future, those formulated endophytes can serve as a good replacement for chemical fertilizers which have a harmful effect on agriculture.

KEYWORDS

• Endophytes
• Solanum lycopersicum
• Fusarium oxysporum
• Samanea saman
• Plant Growth Promotion

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• DOI10.5530/ajbls.2024.13.44Copy

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© 2024 Author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0

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