Studies on in vitro Anti-Diabetic Activities of the Epigeic Earthworm, Eisenia fetida Extract

Abstract

Aim: Diabetes is considered as one of the serious complications and disorders in recent years. So, the present work was undertaken to investigate the anti-diabetic activity using Eisenia fetida earthworm extract.Materials and Methods: The anti-diabetic activity was carried out by using different concentrations of earthworm, Eisenia fetida extract with two different methods: alpha-amylase inhibitory assay and glucose uptake assay, by using standard drugs Metformin and Metronidazole, respectively.Results: Results revealed that the earthworm, Eisenia fetida extract exhibits anti-diabetic activity by inhibiting alpha-amylase activity and also by increasing the glucose uptake in the cells. Thus, Eisenia fetida earthworm extract may serve as an alternative anti-diabetic drug to prevent diabetic complications in the near future.

Keywords: Alpha-amylase assay; Anti-diabetic activity; Eisenia fetida and Glucose uptake assay.