Cytotoxic Activity of Cardiospermum halicacabum L. against Oral Cancer Cell Lines.-SCC25

Abstract

Aim: To evaluate the cytotoxic activity of Cardiospermum halicacabum L. against oral cancer cell lines (SCC25).Method: Oral cancer cells were plated in 96-well flat-bottom tissue culture plates at a density of approximately 1.2 × 104 cells/well and allowed to attach overnight at 37°C. The medium was then discarded, and cells were incubated with different concentrations of the samples (25, 50, 75, 100, and 125 μg/mL) for 24 hours. After the incubation, the medium was discarded, and 100 μl of fresh medium was added with 10 μl of MTT (5 mg/mL). After 4 hrs, the medium was discarded, and 100 μl of DMSO was added to dissolve the formazan crystals. Then, the absorbance was read at 570 nm in a microtiter plate reader. Cyclophosphamide was used as a positive control for the cancer cell lines. Medium along with cells (untreated) served as a control.Results: Our present study showed the cytotoxicity levels of Cardiospermum halicacabum L. in conjunction with a positive control, cyclophosphamide, at different concentrations (25 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL, 125 μg/mL). Cyclophosphamide was chosen as a positive control, as it is known for its cytotoxicity ability in cancer cell lines. The cytotoxicity level seems to increase with an increase in the concentration of the compound. While comparing the test compound with the positive control, the cytotoxicity levels of the test compound were not as efficient as those of cyclophosphamide, but within the compound concentrations, it had good cytotoxicity capacity.Conclusion: Hence, furthermore, investigations with higher concentrations and using this product with other compounds as an adjuvant can give a better result.

Keywords: Cancer cell lines; Cardiospermum halicacabum L; cyclophospahmide; cytotoxicity; Oral cancer