Asian Journal of Biological and Life Sciences logo
Submit
Asian Journal of Biological and Life SciencesACADEMIC PUBLISHING
Article

Isolation and Identification of Bacteria Producing Extracellular Protease from Port Dickson Seashore, Negeri Sembilan and Kelana Jaya Lake, Selangor

1, 2, 1

  1. 1Department of Biotechnology, Faculty of Science, Lincoln University Collage MALAYSIA, Kelana Jaya Campus, No 2, Jalan Stadium SS7/15, Kelana Jaya, 47301, Selangor, MALAYSIA.
  2. 2School of Biological Sciences, University Sains Malaysia, 11800 Minden, Pulau Pinang, MALAYSIA.

CORRESPONDENCE

Aida Baharuddin

Department of Biotechnology, Faculty of Science, Lincoln University Collage MALAYSIA, Kelana Jaya Campus, No 2, Jalan Stadium SS7/15, Kelana Jaya, 47301, Selangor, MALAYSIA.

envelope910@gmail.com

Received: 22-04-2017; Accepted: 20-06-2017.

Volume 6, Issue 2 · pp. 321-329 · 2017 · DOI:

ABSTRACT

This study was conducted to isolate extracellular protease producing bacteria from the Port Dickson seashore and Kelana Jaya Lake using clear zone formation assay on skim milk agar medium. The extracellular protease producing bacteria identifications were based on morphological examination, Gram staining, oxidase test, lactose fermentation test, catalase test, hemolysis test and bioMérieux's API® identification system. Nine isolates, namely S1, S19, S25, L20, L21, L30, L40, L41, L44, exhibited hydrolysis capacity (HC) between 1.5 to 2.3 when incubated at 30°C for 24, 48, and 72 hour periods. The sea isolate, namely S19, exhibited the highest HC after 24, 48, and 72 h at 30ºC. S19 was identified as Bacillus cereus 2 by API 50 CHB/20E. The second-best HC was represented by lake isolate L20 and identified as Pseduomonas aeruginosa by the API 20NE system.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Yahya, A., Samian, M. R., & Baharuddin, A. (2017). Isolation and Identification of Bacteria Producing Extracellular Protease from Port Dickson Seashore, Negeri Sembilan and Kelana Jaya Lake, Selangor. Asian Journal of Biological and Life Sciences, 6(2), 321-329.

    Copyright and licence

    © 2017 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0