Direct Detection of Bacillus ceruse Enterotoxin Gene by Multiplex PCR Kit in Dairy Products
1, 2
- 1Department of Food Science and Technology, Quchan Banch, Islamic Azad University, Quchan, IRAN.
- 2Young Researchers and Elite Club, Quchan Banch, Islamic Azad University, Quchan, IRAN.
CORRESPONDENCE
Samira Amiri
Department of Food Science and Technology, Quchan Banch, Islamic Azad University, Quchan, IRAN.
Received: 03-02-2015; Accepted: 17-04-2015.
Volume 4, Issue 3 · pp. 194-197 · 2015 · DOI:
ABSTRACT
Due to the heat resistance of Bacillus cereus, its potential pathogenic character, the capability to grow in milk and reported diseases upon consumption of dairy products, the organism should be considered as hazardous in pasteurized milk. In this study hblA, hblC and nheB genes was investigated. Enterotoxin producing ability of 25 raw milk and 5 pasteurization milk samples was judged by Multiplex PCR reactions. Results showed that hblA, hblC and nheB genes were found to be in 66.36, 23.33 and 66.56 percent of milk samples, respectively. Also, 16.66 % and 13.33 % of samples were contained hblA+hblC and all three genes, respectively. Our findings indicated that hblA, hblC and nheB genes specific Multiplex PCR can be employed for differentiation of enterotoxigenic B. cereus isolates.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Amiri, S., & Sani, A. M. (2015). Direct Detection of Bacillus ceruse Enterotoxin Gene by Multiplex PCR Kit in Dairy Products. Asian Journal of Biological and Life Sciences, 4(3), 194-197.
Copyright and licence
© 2015 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0