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The Effects of Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis Venoms on Human Cancer Cells

1, 2, 1, 3, 1, 1, 1

  1. 1Research of Development, Queen Saovabha Memorial Institute, Bangkok 10330, THAILAND.
  2. 2Cellular Immunology Laboratory Allergy and Clinical Immunology Unit, Department of Medicine, Faculty of Medicine Chulalongkorn, University, Bangkok 10330, THAILAND.
  3. 3Institute of Biotechnology and Genetic Engineering, Chulalongkorn University Institute Building 3, Phayathai Road, Patumwan, Bangkok 10330, THAILAND.

CORRESPONDENCE

Suchitra Khunsap

Research of Development, Queen Saovabha Memorial Institute, Bangkok 10330, THAILAND.

sthaithumnas@yahoo.com

Received: 13-01-2013; Accepted: 01-02-2013.

Volume 2, Issue 1 · pp. 50-53 · 2013 · DOI:

ABSTRACT

Three snake venoms: Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis were studied for cytotoxic activity and apoptosis on five cancer cell lines; BT474, SW620, KATO-III, Hep-G and ChaGo. Cytotoxic effect was determined by MTT assay and flow cytometry has 2 been used for apoptosis. Various concentrations of three venoms showed cytotoxic against cancer cells as time-dependent. The potent of cytotoxic from C. albolabris venom against cancer cells were: BT474 (2.96 ± 0.44 µg/ml), SW620 (3.32 ± 0.14 µg/ml), KATO-III (3.72 ± 0.11 µg/ml) and Hep-G (3.74 ± 0.43 µg/ml), respectively. C. rhodostoma venom showed cytotoxic to BT474 (3.16 ± 0.69 µg/ml), SW620 (3.5 ± 0.01 µg/ml) and KATO¬ 2 III (3.74 ± 0.37 µg/ml) while the venom of D. siamensis was highly toxic to only ChaGo (0.48 ± 0.04 µg/ml). Apoptotic cell death using PI staining was dose and time dependent.

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    Khunsap, S., Buranapraditkun, S., Suntrarachun, S., Puthong, S., Khow, O., Chulasugandha, P., & Boonchang, S. (2013). The Effects of Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis Venoms on Human Cancer Cells. Asian Journal of Biological and Life Sciences, 2(1), 50-53.

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    © 2013 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0