In vitro Assessment of the Antioxidant and Cytotoxic Activities of Cissus quadrangularis Using HeLa Cells
1, 1, 2, 2, 2, 2, 2, 2
- 1Department of Biotechnology, School of Technology, GITAM University, Vishakhapatnam, Andhra Pradesh, INDIA.
- 2Centre for Biotechnology, University College of Engineering Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
CORRESPONDENCE
Kalyani Chepuri
Centre for Biotechnology, University College of Engineering Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
Received: 10-10-2024; Revised: 14-11-2024; Accepted: 21-12-2024.
Volume 13, Issue 3 · pp. 760-769 · 2024 · DOI: 10.5530/ajbls.2024.13.92
ABSTRACT
Aim: Cervical cancer is a significant global health concern, and usually treated by Cisplatin in combination with radiation therapy, often associated with side effects. Cissus quadrangularishas antioxidant and immune-boosting effects. In view of its therapeutic potential in treating cervical carcinoma, we evaluated the antioxidant and anticancer activities of acetone and methanolic extracts of Cissus quadrangularis.Materials and Methods: The phytochemical profiling of the plant extract was done by GC-MS, antioxidant activity was assessed by FRAP, DPPH, and ABTS assays, and MTT assay was used to study the cytotoxicity of plant extract in HeLa cells.Results: Phytochemical profile comprised pentadecanoic acid, 9,12,15 octadecatrienoic acid, 9,12 octadecanoic acid, methyl stearate, α-amyrin, α-methyl sorboside, β-amyrin, methyl tetradeconate, heptadecanoic acid, stigamasta, diglycerol, which are testified to have antioxidant and anticancer activities. The FRAP, DPPH and ABTS assays demonstrated a strong antioxidant activity in acetone and methanol as the concentration of the extract increased. In the anticancer assays, both acetone and methanol extracts exhibited dose-dependent cytotoxic effects on the HeLa cell lines, with notable inhibition of cell proliferation. In acetone extract, cell viability was 96.21 % in 0.10 mg/mL and 44.67% in 1.60 mg/mL, whereas in methanol extract, cell viability was 89.32% in 0.10 mg/mL and 32.12% in 1.60 mg/mL. The IC50values of acetone and methanolic extracts were 1.33 and 0.97 mg/mL, respectively.Conclusion: The results advocate a prospective role of C. quadrangularis in cervical carcinoma therapy.
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Kontham, G. R., Vutukuru, G. V. K., Chepuri, K., Chittepu, P., Kathuroju, H., Vadakavila, G., Addepally, U., & Vutukuru, S. S. (2024). In vitro Assessment of the Antioxidant and Cytotoxic Activities of Cissus quadrangularis Using HeLa Cells. Asian Journal of Biological and Life Sciences, 13(3), 760-769. https://doi.org/10.5530/ajbls.2024.13.92
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