Study on the Therapeutic Potential of Pimenta dioica Phytocompounds Isolated from Stem Extract and its Analysis through GCMS Profiling

Abstract

Aim: This study aimed to determine the phytochemical constituents present in the various extracts of Pimenta dioica stem through standard qualitative biochemical and GCMS analysis.Materials and Methods: The phytochemical analysis was carried out according to standard procedures. The total phenol and tannin were estimated using the Folin-Ciocalteu method. The profiling of phytoconstituents present in the ethanolic extract of Pimenta dioica was done by Gas Chromatography-Mass Spectrometry (GCMS) analytic technique (Agilent Technologies).Results: The phytochemical screening of Pimenta dioica stem extract revealed the existence of various phytochemicals. The total phenolic and tannin content of the ethanolic extract was higher than the aqueous extract. GCMS profiling indicated the presence of 21 bioactive compounds, including eugenol, 7(1H)-Pteridinone, phenol, 4-(2-propenyl), 8-methylnonanoic acid, methyl ester, and benzeneacetonitrile. Eugenol was the key component.Conclusion: The phytochemical and GCMS profiling of Pimenta dioica stem extract revealed the presence of bioactive constituents with vital therapeutic properties. Hence, the isolation of individual phytochemical compounds may lead to the discovery of a novel therapeutic drug.

Keywords: Pimenta dioica (Allspice); Phytochemicals; Phenol; Tannin; GCMS