Formulation of Wound Healing Hydrogel Using Carbon Nanoparticles Synthesized from Piper longum

Abstract

Aim: Bacterial infection is a significant factor in the complex process of delayed wound healing. The advent of antibiotic resistance in bacterial isolates led to the creation of antimicrobial drugs from new resources. Formulating the hydrogel using carbon nanoparticles synthesized from the burnt ash of Piper longum against the multidrug-resistant skin infection-causing pathogens was the goal of the current study.Materials and Methods: The dried fruit of Piper longum was collected from the local market, burnt, and carbon nanoparticles were synthesized from the burnt ash. The synthesized nanoparticles were characterized, and the antibacterial activity as well as the MIC value were assessed.Results: The absorption peak spectrum, size, and shape were identified by UV-Vis spectrophotometry, Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD). Additionally, the functional groups present in the synthesized carbon nanoparticles were detected by FTIR analysis. The synthesized carbon nanoparticles showed bactericidal action against multidrug-resistant Escherichia coli, Staphylococcus aureus, and K. pneumoniae. Minimum inhibitory concentration, red blood cell hemolysis, time-kill assay, and skin irritation tests were performed.Conclusion: The developed wound-healing hydrogel demonstrated both significant wound healing and antibacterial action. As a result, the formulated hydrogel has the potential to be employed as a dressing for wounds.

Keywords: Anti-bacterial; Carbon nanoparticles; Hydrogel; Multidrug resistance; Piper longum