Effect of Alpha-Phellandrene on Glucose Uptake and Adipogenesis in Insulin Resistant 3T3-L1 Adipocytes: an in vitro and in silico Approach
1, 1, 1
- 1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, INDIA.
CORRESPONDENCE
Chandrasekaran Sankaranarayanan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, INDIA.
Received: 11-09-2023; Revised: 17-10-2023; Accepted: 15-11-2023.
Volume 12, Issue 3 · pp. 603-610 · 2024 · DOI: 10.5530/ajbls.2023.12.79
ABSTRACT
Aim: The present study was designed to investigate the effect of alpha-phellandrene, a monoterpene present in essential oils of mint, turmeric, black pepper, and cinnamon, on adipocyte function under insulin-resistant conditions.Materials and Methods: Mature 3T3-L1 adipocytes were exposed to high glucose (25 mM) and 0.6 nM/L of insulin for 24 hr to convert to IR-3T3-L1 adipocytes. The adipocytes were grouped into group 1: normal control, group 2: diabetic control, group 3: diabetic group treated with alpha-phellandrene (65 μM), and group 4: diabetic group treated with rosiglitazone (0.1 μM). Glucose uptake assay, triglyceride accumulation, and the activity of glycerol-3-phosphate dehydrogenase were measured. The binding affinity of alpha-phellandrene with PPARγ and SREBP-1c was analyzed by docking studies using AutoDock Vina (V. 4.0) in Pyrx software.Results: Glucose uptake, triglyceride accumulation, and activity of glycerol-3-phosphate dehydrogenase were found to be decreased in the diabetic control compared to the normal control group. Alpha-phellandrene at a dose of 65 μM increased glucose uptake, enhanced glycerol-3-phosphate activity, and triglyceride accumulation in adipocytes, which were found to be comparable with the standard drug rosiglitazone. A high binding affinity of alpha-phellandrene with key transcription factors, PPARγ and SREBP-1c, which are associated with adipogenesis and steroidogenesis, was observed in molecular docking studies.Conclusion: These results suggest that alpha-phellandrene positively regulates adipocyte function and ameliorates dyslipidemia and hyperglycemia in type II diabetes mellitus.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Seethalakshmi, S., Sarumathi, R., & Sankaranarayanan, C. (2024). Effect of Alpha-Phellandrene on Glucose Uptake and Adipogenesis in Insulin Resistant 3T3-L1 Adipocytes: an in vitro and in silico Approach. Asian Journal of Biological and Life Sciences, 12(3), 603-610. https://doi.org/10.5530/ajbls.2023.12.79
Copyright and licence
© 2024 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0