Green Synthesis of AgNP Using Ficus benghalensis Aerial Root and its AchE Inhibiting Property

Abstract

Aim: The aim of the study was to analyze the phytochemical components of adventitious roots of Ficus benghalensis and its biopotential ability on silver nanoparticle reduction.Materials and Methods: Aerial root was collected and its phytochemicals were extracted with acetone, and phytochemicals were detected by GCMS. AgNP was characterized by UV and SEM analysis. DPPH antioxidant and AchE inhibition were performed.Results: GCMS revealed 20 different peaks, with 19 different compounds, predominantly with 2-Lauro-1,3-Didecoin, Alpha. Tocospiro A, and 2h-1,2,3-Triazole-4-Carboxylic Acid, 2-(2-Fluorophenyl). The extract reduced silver at a 1:9 ratio, and the time frame was recorded at 5 minutes to produce nanoparticles. The UV-vis absorption spectra show the absorbance peak in the range of 430 nm, and SEM reveals the size of AgNP was 62 nm in rectangle shapes. The free radical scavenging activity of the hydroalcoholic extract of F. benghalensis root was determined and found to have more effective antioxidant activity than the standard. AchE inhibition was moderate on the root extract and significantly inhibited by AgNP.Conclusion: Green silver nanoparticles reduced by aerial root and its antioxidant potential, along with inhibition of Acetylcholine esterase, is still considered the main therapeutic strategy against Alzheimer’s Disease. The study plant F. benghalensis will improve cognitive function and was screened for acetylcholinesterase inhibitory activity with nano drug delivery, also a better candidate for future disease-modifying therapies against this devastating disease.

Keywords: Acetylcholinesterase; AgNP; F. benghalensis; UV-vis