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AJBLS · Lens · Evaluation of Anticancer Activity of Ethanolic Extract of Strobilanthes ciliatus Leaves on HT–29 Cell Lines

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Article · Volume 12, Issue 1 · pp. 73-79

Evaluation of Anticancer Activity of Ethanolic Extract of Strobilanthes ciliatus Leaves on HT–29 Cell Lines

Rani Sebastian1, Gomathi Venkatachalam2, Umamaheswari D3, Sakthi Abirami M4, Venkateswarlu BS5

  1. 1Vinayaka Mission’s College of Pharmacy, Yercad Main Road, Salem, Vinayaka Mission’s Research Foundation (Deemed to be University), Ariyanur, Salem, Tamil Nadu, INDIA.
  2. 2Department of Pharmacology, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed to be University), Ariyanur, Salem, Tamil Nadu, INDIA.
  3. 3Department of Pharmaceutical Analysis, Vinayaka Mission’s College of Pharmacy, Salem, Vinayaka Mission’s Research Foundation (Deemed to be University), Ariyanur, Salem, Tamil Nadu, INDIA.
  4. 4Department of Pharmacology, Madras Medical College, Chennai, Tamil Nadu, INDIA.
  5. 5Vinayaka Mission’s College of Pharmacy, Yercad Main Road, Salem, Tamil Nadu, INDIA.

Abstract

Aim: The present research is aimed at highlighting the cytotoxic potential of Strobilanthes ciliatus on HT-29 cell lines.Materials and Methods: Extraction was done with ethanol (SCLE) and acetone (SCLA) by the Soxhlet method, and anticancer activity was assessed by MTT assay. GCMS was used to analyze the phytochemicals of the plant, and MTT flow cytometry was used to assess cytotoxicity.Results: A total of 38 distinct compounds were found in this study's analysis of the plant's entire chemical profile, and three of those compounds were found in two different solvent extractions. LD was assessed using the MTT and flow cytometry methods, and the transmittance of the extraction-treated HT29 cells was determined using a plate reader at a wavelength of 570 nm. SCLE had the largest antiproliferative effect of the two investigated extractions, with an IC50 value of 33.62 g/mL, while the acetone extract had less impact.Conclusion: The results showed significant cytotoxic activity only with ethanol extraction, not with acetone extract, due to the concentration and diversity of compounds. The studies reveal that the natural plant extract showed effective anticancer activities in vitro that can be further subjected to the development of a potential therapeutic anticancer agent.

Keywords: Anticancer; Anticancer activity; Cytotoxic.; cytotoxicity; HT-29 cell lines; MTT assay; Strobilanthes ciliatus IC50

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