Studies on Mycorrhizal Associations in an Orchid
1
- 1Department of Bioscience and Technology, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, INDIA.
CORRESPONDENCE
Lucky Kumar Attri
Department of Bioscience and Technology, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, INDIA.
Received: 15-02-2023; Revised: 14-03-2023; Accepted: 26-03-2023.
Volume 12, Issue 1 · pp. 179-186 · 2023 · DOI: 10.5530/ajbls.2023.12.24
ABSTRACT
Background: Known for their myriad of shapes, sizes, and colors, orchids embody an air of aristocracy among flowering plants and are among the top ten floriculturally important flowering plants in the international market. Worth noting is that for growth and development, the mycorrhizal association plays an important part in orchids; therefore, we want to trace the structure, pattern of infestation, growth pattern, and genus involved in mycorrhiza.Aim: The present work is based on mycorrhizal association in Cymbidium pendulum (Roxb.), an orchid plant, with a stress on the extent of infusion, fate, isolation, and identification of fungal partners.Materials and Methods: Cymbidium pendulum, an epiphytic plant, is an important medicinal orchid. It had been collected from its natural habitat, Kanchenjunga hills, and was maintained in the orchid house, Botanical Garden, Panjab University, Chandigarh, India, for further use. After section cutting, observations were recorded. Chemically defined MOM and MS and undefined PDA, DPA, PCACMA, and MEAB media were used as sources of nutrition in vitro. Fungal cultures were raised using standard methods and stored at 4°C until use.Results: After sectioning, it was observed that the fungal partner was established to enter the cortex. Entry of the fungal partner in the course of roots is mediated through the thin-walled ‘passage cells.’ Further, the fungal symbiont was observed to be anamorphs of Rhizoctonia species when processed with cotton blue, after characterization based on their morphological and growth characteristics on different media. PDA and MS, followed by Mitra media, were studied to be more fruitful for fungal growth.Conclusion: The fungal endophytes have been isolated from the different isolated media, and their pure cultures were obtained on PDA medium. The fungal partner was established to enter the cortex. The access of the fungal symbiont through roots was mediated via the thin-walled ‘passage cells.’ The fungal endophyte was identified on the basis of morphological and its growth characteristics; it was found to belong to the genus Rhizoctonia.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Attri, L. K. (2023). Studies on Mycorrhizal Associations in an Orchid. Asian Journal of Biological and Life Sciences, 12(1), 179-186. https://doi.org/10.5530/ajbls.2023.12.24
Copyright and licence
© 2023 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0