Nitrogen fixation by the Native Azospirillum spp. Ssolated from Rhizosphere and Non-Rhizosphere of foxtail Millet
1, 1
- 1Department of Microbiology, Sri Krishnadevaraya University, Anantapur - 515003, INDIA.
- 2Department of Biotechnology, Sri Krishnadevaraya University, Anantapur-515003, INDIA.
CORRESPONDENCE
Mokula Md. Raffi
Department of Microbiology, Sri Krishnadevaraya University, Anantapur - 515003, INDIA.
Received: 13-07-2012; Accepted: 11-08-2012.
Volume 1, Issue 3 · pp. 213-218 · 2012 · DOI:
ABSTRACT
Experiments were conducted to isolate Azospirillum spp. from the rhizosphere and non-rhizosphere soil of foxtail millet grown in native black loamy soils of Anantapur district. A total of 100 strains of Azospirillum were isolated and grown on semi-solid nitrogen-free bromothymol blue malate semisolid malate agar (Nfb), purified and tested for nitrogen fixing abilities, only 56 strains of Azospirillum spp. strains showed nitrogen fixing ability, -1 out of which 20 strains fixed nitrogen efficiently. Azospirillum strains varied in their nitrogen-fixing abilities ranging from 2.95 to 12.4 mg g malate. All the strains were identified as Azospirillum lipoferum. These strains when tested for IAAproduction, all produced IAA.
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Raffi, M. M., & Charyulu, P. (2012). Nitrogen fixation by the Native Azospirillum spp. Ssolated from Rhizosphere and Non-Rhizosphere of foxtail Millet. Asian Journal of Biological and Life Sciences, 1(3), 213-218.
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